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The modification in functional groups present in the culture pellets was identified by measuring the spectra in the range of 400 to 4000 cm -1 using Fourier transform infrared (FT-IR) spectroscopy (IR Affinity-I spectrometer, Shimadzu, Japan), as described by Joshi etal. (2021). In brief, 48 h grown cultures in ZMB medium without Hg supplementation were used as a control, whereas cultures with 50 mg/L of Hg supplementation were used as Hg treated. The mixtures (lyophilized cells and 2% KBr) were fixed in the FT-IR spectrometer after compressing them into translucent sample discs, followed by analyzing in ATR-FT-IR mode by following the manufacturer’s protocol. Scanning Electron Microscopy (SEM) analysis of MRB It can be observed that high Hg concentration causes structural deformities which may be due to its toxic nature. In our previous study, we reported that cultures accustomed to grow in the presence of HgCl 2 manifested major morphological abnormalities ( Joshi etal., 2021). We also suggested several structural asymmetries allied with the cell wall and cytoplasmic membrane, significantly affecting the cellular mechanism. Resultantly, there is a substantial delay at the beginning of growth and cell division in a higher concentration of Hg 2+. Metabolites formed during Hg (II) reduction

Gajendra Joshi 1* Pankaj Verma 2 Balakrishnan Meena 1 Prasun Goswami 1† D Magesh Peter 2 Dilip Kumar Jha 2 Nambali Valsalan Vinithkumar 1 Gopal Dharani 2 The health effects of alcohol intake are variable and are based on the amount of intake (low, moderate, or heavy) and intake pattern (acute, chronic, or binge) [ 45- 47]. In the US, the amount of pure alcohol in one standard drink is 14 g, which can be found in 12 oz of regular beer (about 5% alcohol), 5 oz of wine (about 12% alcohol), and 1.5 oz of distilled spirits (about 40% alcohol) [ 48]. Moderate alcohol intake recommendations according to the Dietary Guidelines for Americans are the consumption of two standard drinks per day for men and one standard drink per day for women [ 49]. Moderate alcoholic intake has been shown to reduce the risk of chronic disease [ 49]. The selected isolates were further analyzed by subjecting them to 16S rDNA sequencing. In brief, DNA was isolated and the polymerase chain reaction (PCR) amplification was executed with the universal 16S rDNA primers of 27F (5’-AGAGTTTGATCMTGGCTCAG-3’) and 1495R (5’-GGHTACCTTGTTACGACTT-3’) as described by Dash and Das (2014). The amplified PCR products were sequenced and submitted to NCBI, as described by Kumar etal. (2018). The detailed procedure is elaborated in the SI. Genotyping of merA gene In our recent study ( Joshi etal., 2021), we isolated and characterized several MRBs from the deeper depth of the Central Indian Ocean and evaluated their ability to remove Hg from the culture media. However, no bacterial strain was observed from the surface seawater samples that were resistant to Hg up to 100 mg/L. Though various bacterial strains have been isolated from the coastal and oceanic region, so far. MRB from the equatorial region of the Indian Ocean (ERIO) is not studied. It is hypothesized that the marine bacteria from ERIO could be a potential resource for the reduction of Hg 2+ as ERIO is highly dynamic in nature due to high current and intense climatological precipitation ( Annamalai, 2010).HG car dashboard cleaner makes the interior of the car look like new. This dash cleaner cleans and protects synthetic materials, plastic, rubber, vinyl and leather. Also, the car dash cleaner intensifies the colour and restores the shine of the polished surface. The HG dashboard spray also leaves a wonderfully fresh fragrance after the treatment, but not a detectable greasy coating. How do you use HG car dashboard cleaner? Now the top of the interior is clean, it's time to shake and beat the mats. That will deal with the larger bits of dirt.

Based on phenotype characteristics, a total of 162 bacterial colonies grown on the ZMA media supplemented with 10 mg/L of Hg as HgCl 2 were selected for further analysis. In the presence of 25 mg/L of Hg 2+, only 63 isolates out of 162 showed resistance. Further, these 63 isolates were characterized based on their growth pattern in the presence of more than 25 mg/L i.e. 50, 75, and 100 mg/L of Hg 2+ concentration. Among the 63 isolates, 21 isolates grew in the presence of 50 mg/L, followed by 9 and 4 isolates at 75 and 100 mg/L of HgCl 2, respectively. The growth pattern of 4 isolates in the presence of HgCl 2 is shown in Figure S1. Assorted studies have shown that IF lowers BP. Harvie et al., in their study involving 107 overweight or obese premenopausal women, showed that IF for six months helped lower SBP (p= 0.99) and DBP (p= 0.84) [ 22]. Varady et al., in their study performed for 12 weeks with IF involving 15 overweight individuals (five males, 10 females with a BMI of 20-29.9 kg/m 2 showed that IF helped lower BP with a p-value of 0.51 [ 23]. A study by Bhutani et al. involving 83 obese individuals (three males and 80 females) with a BMI of 30-39.9 kg/m 2revealed that 12 weeks of IF helped lower SBP (p = 0.254) and DBP (p = 0.570) [ 24]. In a study by Eshghinia et al., 15 overweight or obese women with a BMI ≥25 kg/m 2 who followed IF for eight weeks showed a lowering of SBP (p<0.001) and DBP (p<0.05) [ 25]. Teng et al., in their 12 weeks IF study with 28 Malay men with a BMI of 23-29.9 kg/m 2,showed a lowering in SBP (p<0.05) and DBP (p<0.05) [ 26]. Erdem et al., in their study involving 60 participants from the Cappadocia cohort with pre-HTN and HTN with SBP of 120-139 and more than or equal to 140 mm Hg, DBP of 80-80 and more than or equal to 90 mm Hg, revealed that IF helps lower SBP (p<0.001) and DBP (p<0.039) [ 27]. Increased physical activity has been advocated as the first-line intervention for preventing and treating patients with prehypertension and as a treatment strategy for patients with stage 1 or stage 2 HTN, according to the Duthe American College of Sports Medicine, the United States Joint Nations Committee on Prevention, Detection, Evaluation, and Treatment of High BP, the World Health Organization and International Society of Hypertension, and The National Heart Foundation [ 36]. Exercise can consequently help prevent prehypertension from progressing and can help reduce or stop medications prescribed for the treatment of stage 1 HTN [ 37].

Acknowledgments

It will have your employee’s name as held in the Payroll system, associated with this 'HG' account: Manual entry code method Hg volatilization by bacteria involves the reduction of Hg 2+ via the mer system to Hg 0, which is less toxic. It is appealing from the essential and practical viewpoints that halophilic bacteria can also grow in the presence of a higher amount of Hg and volatilized Hg efficiently similar to the non-halophilic bacteria. The ICP-MS analysis did not detect significant Hg loss in the control sample (without bacteria), which confirms the volatility of Hg being biotic ( Table S2). Thus, our results suggested that halophilic bacteria can also remove the Hg from the growth media, indicating that it may be potentially applied in contaminated environments. The Hg comes into the ocean either through atmospheric deposition or other several natural and active or passive processes. The results of this study imply that the two marine strains of Halomonas sp., isolated from ERIO may have the ability to remove Hg 2+ from the growth medium. Identification of functional groups changed during Hg(II) reduction In prior studies, many pathways have been reported for Hg 2+ detoxification in microbes using various tools and technologies ( Chang etal., 2020; Cursino etal., 2000; Chang etal., 2021). In the present study, a metabolic pathway is hypothesized based on the GC-MS metabolites and protein-ligand interaction ( Figure S6) and an attempt has been made to correlate the proposed pathway with the different components of the already existing pathways. In this preliminary study, we found that general resistance/detoxification mechanisms of NIOT-EQR_J248 and NIOT-EQR_J251 in response to inorganic mercury (Hg 2+) exposure were a multisystem combined process. When the isolate NIOT-EQR_J7 was grown in the presence of Hg 2+ supplementation, a slight reduction in the peak intensity attributed to the thiol group (S-H stretch) at 2383.09 cm -1 was observed as compared to the control along with the wavelength that shifted to 2442.42 cm -1. In contrast, the peak intensity corresponding to the thiol group of NIOT-EQR_J248 at 2304.55 cm -1 increased compared to without Hg 2+ supplementation and the shift in wavelength to 2246.72 cm -1 was observed. In the case of NIOT-EQR_J251, the peaks corresponding to the S-H group appeared at 2341.34 cm -1 in the presence of Hg 2+ whereas, in the absence of Hg 2+, the peak was negligible. The peak was absent in both the presence and absence of Hg 2+ in the case of isolate NIOT-EQR_J258 ( Figures5A–D). In accord with our findings, previous studies had also reported similar results ( Dash and Das, 2014; De etal., 2014; Joshi etal., 2021) and suggested significant changes in the FT-IR spectrum such as a shift in wavenumber in the region of O-H groups, –S-H group, amide C=O, Nitrile C–N stretch, phosphate groups, etc. The changes in peak height and area have also been observed when isolates were grown in the presence of Hg 2+ ions compared to the controlled ones. The transmittance of the infrared (IR) in the presence of Hg 2+ was lower than in the absence of Hg 2+. The changes suggested that the presence of metal leads to a lesser degree of bond stretching, consequently reducing the IR transmittance. The contribution of functional groups in metal binding was legitimized undeniably by the formation of varying spectral bands with and without Hg ( Oves etal., 2013).